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The reconstitution ofpharaonis phoborhodopsin was performed by incubation of its opsin with 13-cis-retinal. Spectrum change was very slow, and two phases of the change were observed: the first and second phases are due to the transient formation of 13-cis pigment and spontaneous isomerization toall-trans-retinal, respectively. Slow binding supports an idea that the retinal binding pocket of ppR is...
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