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The nitrite reductase (NIR) from Pseudomonas aeruginosa (NIR-Pa) is a soluble enzyme catalysing the reduction of nitrite (NO 2− ) to nitric oxide (NO). The enzyme is a 120kDa homodimer, in which the monomers carry a c-heme domain and a d 1 -heme domain. The structures of the enzyme in both the oxidised and reduced state were solved previously and indicate His327 and His369 as...
Background: Nitrite reductase from Pseudomonas aeruginosa (NiR-Pa) is a dimer consisting of two identical 60 kDa subunits, each of which contains one c and one d 1 heme group. This enzyme, a soluble component of the electron-transfer chain that uses nitrate as a source of energy, can be induced by the addition of nitrate to the bacterial growth medium. NiR-Pa catalyzes the reduction of nitrite...
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