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The effect of Wnt10b overexpression on adipose tissue development has been reported. However, the impact of Wnt10b knockdown on the function of brown adipose tissue (BAT) is yet largely unknown. Here, we used the CRISPR/Cas9 technique to generate Wnt10b‐knockdown (Wnt10b+/−) mice. We compared the development and thermogenic gene expression of interscapular BAT (iBAT) between Wnt10b+/− and Wnt10b+/+...