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Binding of the bis-intercalators echinomycin and N,N'-di(9-acridinyl)spermidine or the mono-intercalators 9-aminoacridine and ethidium to DNA induces hypersensitivity of adenines towards reaction with diethyl pyrocarbonate. It is proposed that this hyperreactivity is due to the DNA helix unwinding and extension induced by intercalation, thereby exposing N7 in the major groove, and not as previously...
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