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For ultrasensitive bioaffinity assays, the label technology employed should produce intense signals upon specific binding of the label bioconjugate to analyte. Normally, signal levels are improved by optimizing the detection conditions of the label and by utilizing special signal enhancement methods. We have now discovered unexpectedly strong signal enhancement for up-converting (UPC) phosphors in...
We have characterized commercially available up-converting inorganic lanthanide phosphors for their rare earth composition and photoluminescence properties under infrared laser diode excitation. These up-converting phosphors, in contrast to proprietary materials reported earlier, are readily available to be utilized as particulate reporters in various ligand binding assays after grinding to submicron...
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