Wyniki wyszukiwania dla: Patrick S. Stayton

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Nanostructured glycopolymer augmented liposomes to elucidate carbohydrate-mediated targeting

Jasmin Chen, Hye-Nam Son, John J. Hill, Selvi Srinivasan, więcej

Nanomedicine: Nanotechnology, Biology and Medicine > 2016 > 12 > 7 > 2031-2041

Carbohydrate receptors on alveolar macrophages are attractive targets for receptor-mediated delivery of nanostructured therapeutics. In this study, we employed reversible addition fragmentation chain transfer polymerization to synthesize neoglycopolymers, consisting of mannose- and galactose methacrylate-based monomers copolymerized with cholesterol methacrylate for use in functional liposome studies...

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Theranostic Oxygen Reactive Polymers for Treatment of Traumatic Brain Injury

Julia Xu, Menko Ypma, Peter A. Chiarelli, Joshua Park, więcej

Advanced Functional Materials > 26 > 23 > 4124 - 4133

Traumatic brain injury (TBI) is the leading cause of disability and death in children and adults under 45, with approximately ten million new cases per year worldwide. Significant progress has been made in understanding the complex pathophysiological response to TBI; however, reducing the damage associated with the reactive oxygen species (ROS)‐dependent secondary phase of the injury remains a substantial...

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Editorial

Suzie H. Pun, Patrick S. Stayton

Journal of Controlled Release > 2016 > 231 > C > 1

artykuł

Polymer nanostructures synthesized by controlled living polymerization for tumor-targeted drug delivery

Christine E. Wang, Patrick S. Stayton, Suzie H. Pun, Anthony J. Convertine

Journal of Controlled Release > 2015 > 219 > C > 345-354

The development of drug delivery systems based on well-defined polymer nanostructures could lead to significant improvements in the treatment of cancer. The design of these therapeutic nanosystems must account for numerous systemic and circulation obstacles as well as the specific pathophysiology of the tumor. Nanoparticle size and surface charge must also be carefully selected in order to maintain...

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Enhancement of MHC-I Antigen Presentation via Architectural Control of pH-Responsive, Endosomolytic Polymer Nanoparticles

John T. Wilson, Almar Postma, Salka Keller, Anthony J. Convertine, więcej

The AAPS Journal > 2015 > 17 > 2 > 358-369

Protein-based vaccines offer a number of important advantages over organism-based vaccines but generally elicit poor CD8⁺ T cell responses. We have previously demonstrated that pH-responsive, endosomolytic polymers can enhance protein antigen delivery to major histocompatibility complex class I (MHC-I) antigen presentation pathways thereby augmenting CD8⁺ T cell responses following immunization. Here,...

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Neutral polymer micelle carriers with pH-responsive, endosome-releasing activity modulate antigen trafficking to enhance CD8+ T cell responses

Salka Keller, John T. Wilson, Gabriela I. Patilea, Hanna B. Kern, więcej

Journal of Controlled Release > 2014 > 191 > Complete > 24-33

Synthetic subunit vaccines need to induce CD8 ⁺ cytotoxic T cell (CTL) responses for effective vaccination against intracellular pathogens. Most subunit vaccines primarily generate humoral immune responses, with a weaker than desired CD8 ⁺ cytotoxic T cell response. Here, a neutral, pH-responsive polymer micelle carrier that alters intracellular antigen trafficking was shown to enhance...

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A Computationally Designed Inhibitor of an Epstein-Barr Viral Bcl-2 Protein Induces Apoptosis in Infected Cells

Erik Procko, Geoffrey Y. Berguig, Betty W. Shen, Yifan Song, więcej

Cell > 2014 > 157 > 7 > 1644-1656

Because apoptosis of infected cells can limit virus production and spread, some viruses have co-opted prosurvival genes from the host. This includes the Epstein-Barr virus (EBV) gene BHRF1, a homolog of human Bcl-2 proteins that block apoptosis and are associated with cancer. Computational design and experimental optimization were used to generate a novel protein called BINDI that binds BHRF1 with...

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Functionalized nanoparticles provide early cardioprotection after acute myocardial infarction

Ming-Yao Chang, Yu-Jen Yang, Chih-Han Chang, Alan C.L. Tang, więcej

Journal of Controlled Release > 2013 > 170 > 2 > 287-294

Recent developments in nanotechnology have created considerable potential toward diagnosis and cancer therapy. In contrast, the use of nanotechnology in tissue repair or regeneration remains largely unexplored. We hypothesized that intramyocardial injection of insulin-like growth factor (IGF)-1-complexed poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles (PLGA-IGF-1 NPs) increases IGF-1 retention,...

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Melittin-grafted HPMA-oligolysine based copolymers for gene delivery

Joan G. Schellinger, Joshuel A. Pahang, Russell N. Johnson, David S.H. Chu, więcej

Biomaterials > 2013 > 34 > 9 > 2318-2326

Non-viral gene delivery systems capable of transfecting cells in the brain are critical in realizing the potential impact of nucleic acid therapeutics for diseases of the central nervous system. In this study, the membrane-lytic peptide melittin was incorporated into block copolymers synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization. The first block, designed for...

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pH‐Responsive Hyperbranched Copolymers from One‐Pot RAFT Copolymerization

Hongyun Tai, Craig L. Duvall, Alan S. Hoffman, Patrick S. Stayton, więcej

Macromolecular Materials and Engineering > 297 > 12 > 1175 - 1183

The synthesis of new pH‐responsive hyperbranched copolymers via one pot RAFT copolymerization of PAA and a branching co‐monomer PEGDA ($\overline {M} _{{\rm n}} $ = 258 Da) is reported. Three copolymers are prepared at monomer feed molar ratios [PAA]₀/[PEGDA]₀ = 99/1, 90/10, and 80/20. The resultant poly(PAA‐PEGDA) copolymers are characterized by ¹H NMR, GPC, and DLS. These hyperbranched copolymers...

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Multifunctional triblock copolymers for intracellular messenger RNA delivery

Connie Cheng, Anthony J. Convertine, Patrick S. Stayton, James D. Bryers

Biomaterials > 2012 > 33 > 28 > 6868-6876

Messenger RNA (mRNA) is a promising alternative to plasmid DNA (pDNA) for gene vaccination applications, but safe and effective delivery systems are rare. Reversible addition-fragmentation chain transfer (RAFT) polymerization was employed to synthesize a series of triblock copolymers designed to enhance the intracellular delivery of mRNA. These materials are composed of a cationic dimethylaminoethyl...

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Diblock copolymers with tunable pH transitions for gene delivery

Matthew J. Manganiello, Connie Cheng, Anthony J. Convertine, James D. Bryers, więcej

Biomaterials > 2012 > 33 > 7 > 2301-2309

A series of diblock copolymers containing an endosomal-releasing segment composed of diethylaminoethyl methacrylate (DEAEMA) and butyl methacrylate (BMA) were synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization. The materials were designed to condense plasmid DNA (pDNA) through electrostatic interactions with a cationic poly(N,N-dimethylaminoethyl methacrylate) (DMAEMA)...

artykuł

RAFT-synthesized graft copolymers that enhance pH-dependent membrane destabilization and protein circulation times

Emily Crownover, Craig L. Duvall, Anthony Convertine, Allan S. Hoffman, więcej

Journal of Controlled Release > 2011 > 155 > 2 > 167-174

Here we describe a new graft copolymer architecture of poly(propylacrylic acid) (polyPAA) that displays potent pH-dependent, membrane-destabilizing activity and in addition is shown to enhance protein blood circulation kinetics. PolyPAA containing a single telechelic alkyne functionality was prepared via reversible addition–fragmentation chain transfer (RAFT) polymerization with an alkyne–functional...

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Synthesis of Folate-Functionalized RAFT Polymers for Targeted siRNA Delivery

Danielle S. W. Benoit, Selvi Srinivasan, Andrew D. Shubin, Patrick S. Stayton

Biomacromolecules > 2011 > 12 > 7 > 2708 - 2714

artykuł

Efficient intracellular delivery of a pro-apoptotic peptide with a pH-responsive carrier

Brian Albarran, Allan S. Hoffman, Patrick S. Stayton

Reactive and Functional Polymers > 2011 > 71 > 3 > 261-265

A key challenge in developing protein therapeutics or imaging agents that work against cytosolic targets is the intracellular delivery barrier. Here, we show that the pH-responsive, membrane-destabilizing polymer, poly(propylacrylic acid) (PPAA), can strongly enhance target cell killing through the intracellular delivery of a functional pro-apoptotic peptide. The Bak BH3 peptide induces apoptosis...

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Delivery of basic fibroblast growth factor with a pH-responsive, injectable hydrogel to improve angiogenesis in infarcted myocardium

Jessica C. Garbern, Elina Minami, Patrick S. Stayton, Charles E. Murry

Biomaterials > 2011 > 32 > 9 > 2407-2416

A pH- and temperature-responsive, injectable hydrogel has been designed to take advantage of the acidic microenvironment of ischemic myocardium. This system can improve therapeutic angiogenesis methods by providing spatio-temporal control of angiogenic growth factor delivery. The pH- and temperature-responsive random copolymer, poly(N-isopropylacrylamide-co-propylacrylic acid-co-butyl acrylate) (p[NIPAAm-co-PAA-co-BA]),...

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ToF‐SIMS imaging and depth profiling of HeLa cells treated with bromodeoxyuridine

Jeremy Brison, Danielle S. W. Benoit, Shin Muramoto, Michael Robinson, więcej

Surface and Interface Analysis > 43 > 1‐2 > 354 - 357

Time‐of‐flight (ToF) SIMS 2D images and molecular depth profiles of human HeLa cells treated with bromodeoxyuridine (BrdU) were acquired in the dual beam mode (Bi₃⁺ analysis beam, C₆₀⁺ etching beam). Several preparation protocols were investigated and were compared to a simple wash‐and‐dry method. The feasibility of using C₆₀ to clean the samples prior to imaging with Bi was also investigated quantitatively...