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The high background signal caused by non-specific amplification (NSA) is a serious issue when using the conventional exponential amplification reaction (EXPAR). We describe a novel method of suppressing NSA using 10 mg L −1 graphene oxide (GO) to prevent non-specific binding of DNA polymerase to the template, resulting in ultra-low background. A side effect of the use of GO is the...
Conventional pyrosequencing using 2′-deoxyadenosine-5′-O-(1-thiotriphosphate) (dATPαS) is problematic due to the high cost of the substrate (dATPαS) and deterioration in the accuracy of incorporation to read through poly(T) regions. One reason for these problems is that dATPαS has a sulfur on the α-phosphate and also has isomers (Sp and Rp). To solve these problems, 11 nucleotide substrates, which...
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