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Ribonuclease T 1 (RNase T 1 ) carboxymethylated at the γ-carboxyl group of Glu-58 with iodoacetic acid is known to be completely inactive while it retains an almost full substrate-binding ability. In order to further clarify the effects of the carboxymethylation, the thermal stabilities of intact and Glu-58-carboxymethylated (CM-) RNase T 1 were compared by measuring 1...
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