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Sulfated oligosaccharides are an important class of compounds in the field of glycobiology. Mass spectrometric analysis of these molecules is challenging due to their readiness to dissociate in sample preparation and their tendency to fragment during ionization. Moreover, their presence in small quantity in biological systems poses additional problems. We report the development of a mass spectrometric...
All six isomeric mono-O-sulfates of β-d-Gal p-(1 → 4)-β-d-Glc pNAc-O-(CH 2 ) 8 COOMe (LacNAc-MCO) have been chemically synthesized and characterized by high resolution 1 H NMR spectroscopy. Sulfation causes characteristic substitution-site-specific downfield shifts of 1 H NMR signals. The 4 C 1 chair conformation of both pyranose residues of LacNAc...
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