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In this study, a novel kind of amide functionalized hydrophilic monolith was synthesized by the in situ photo-polymerization of N-vinyl-2-pyrrolidinone (NVP), acrylamide (AM), and N, N’-methylenebisacrylamide (MBA) in a UV transparent capillary, and successfully applied for hydrophilic interaction chromatography (HILIC) based enrichment of N-linked glycopeptides. With 2μg of the tryptic digests of...
The large‐scale N‐glycosylation analysis is critical for biomedical research, since a variety of diseases are found to be associated with glycoproteins. By a combination of glycoprotein analysis in insoluble protein fraction solubilized with 1% v/v 1‐butyl‐3‐methylimidazolium tetrafluoroborate (BMIM BF4) and those in soluble fraction, a total number of 462 non‐redundant N‐glycoprotein groups, including...
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