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The applicability of two-photon excitation (TPE) for fluorescence detection in flow dynamic systems was explored. Emphasis was on conventional-size liquid chromatography (LC) and a direct comparison was made with one-photon excitation (OPE) by the use of standard laser- and lamp excitation. Simultaneous two-photon excitation (STPE) with visible laser light was used for fluorescence detection of UV-absorbing...
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