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An electric field is focused on one cell in single-cell electroporation. This enables selective electroporation treatment of the targeted cell without affecting its neighbors. While factors that lead to membrane permeation are the same as in bulk electroporation, quantitative description of the single-cell experiments is more complicated. This is due to the fact that the potential distribution cannot...
Electroporation of single NG108-15 cells with carbon-fiber microelectrodes was characterized by patch-clamp recordings and fluorescence microscopy. To minimize adverse capacitive charging effects, the patch-clamp pipette was sealed on the cell at a 90o angle with respect to the microelectrodes where the applied potential reaches a minimum. From transmembrane current responses, we determined the electric...
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