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A reagentless biosensor for d-lactate was developed using the screen-printing technology. In a simple design, d-lactate dehydrogenase and NAD + were deposited onto the surface of a carbon electrode, modified with an insoluble salt of Meldola's Blue. The detection of d-lactate was performed by chronoamperometry and took advantage of the constant potential oxidation of NADH, formed in the reaction...
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