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The role of protein kinase C (PKC) was examined in the regulation of dopamine transport in C6 glioma cells stably expressing the human dopamine transporter. The PKC activating phorbol esters phorbol 12-myristate 13-acetate (PMA) and 4β-12,13-dibutyrate phorbol-ester (PDBu) inhibited [ 3 H]dopamine uptake concentration dependently. These effects were attenuated by the PKC inhibitor staurosporine...
Translocation of [ 3 H]dopamine and binding of 2 β-carbomethoxy-3 β-(4-fluorophenyl)[ 3 H]-tropane ([ 3 H]WIN 35,428) were measured in crude synaptosomal preparations from rat striatum under identical conditions of assay buffer (phosphate-Krebs) and temperature (25°). [ 3 H]Dopamine uptake as a function of time was close to linear for at least 8 min, whereas [ ...
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