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Acysteine proteinase inhibitor has been purified from immature fruit of Malusdomestica (var. Royal Gala). The M r of this apple cystatin is estimated to be 10 700 by MALDI-TOF mass spectrometry, 11 300 by SDS-PAGE and 11 000 by gel filtration. It is a relatively strong inhibitor of papain with a K i value of 0.21 nM and also inhibits ficin and bromelain but not cathepsin B. An amino...
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