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The spin label ESR technique was used to monitor the hydration effect on the molecular dynamics of lipids at three depths of the alkyl chain in stratum corneum (SC) membrane. Increase in water content of neonatal rat SC leads to an increase in membrane fluidity, especially in the region near the membrane-water interface. The reorientational correlation time at the C-16 position of hydrocarbon chains...
Purpose: This study is devoted to a comparison of the effects of diacylglycerols (DAG) and ceramides in bilayer destabilization and fusion.Methods: We have studied the catalytic action of phospholipase C (PLC) and sphingomyelinase (SMase), on large unilamellar vesicles (LUVs) consisting of PC:PE:CH or SM:PE:CH.Results: When SM-containing LUVs are treated with SMase, production of ceramides in the...
Purpose: We have studied the effect of different glycoshingolipids on the liposome fusion induced by phospholipase C.Methods: We used fluorescence methods to monitor lipid mixing and fusion. We employed 31 P-NMR and DSC for structural studies.Results: We found that gangliosides exert a dual inhibitory effect on the events induced by PLC on PC/PE/Chol (2:1:1) LUVs. On the one hand, these...
Purpose: Surfactant interaction with erythrocytes is complex. Both cell protection and cell lysis are observed, at different concentrations. We examined the effect of hydrophyllic chain length of surfactants in the Renex series.Methods: Three nonylphenyl polyoxyethylene [NP(EO) n , Renex] surfactants were used, with n = 9.5, 20 and 100. Surfactant-red cell and ghost interactions were observed...
Purpose: We tried to explain the origin of the latency period found when phospholipase C is hydrolyzing phosphatydilcholine LUVs.Methods: We followed PLC activity by measuring phosphorus contents. Vesicle aggregation was followed by turbidimetry.Results: When acting on PC LUVs, PLC kinetics present a latency period before a [ldquo ]burst[rdquo ] in enzymatic activity. Simultaneous recordings of enzyme...
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