Search results for: Petra Henklein

Items from 1 to 20 out of 21 results

article

ER‐aminopeptidase 1 determines the processing and presentation of an immunotherapy‐relevant melanoma epitope

Kathrin Textoris‐Taube, Clemens Cammann, Petra Henklein, Eylin Topfstedt, more

European Journal of Immunology > 50 > 2 > 270 - 283

Dissecting the different steps of the processing and presentation of tumor‐associated antigens is a key aspect of immunotherapies enabling to tackle the immune response evasion attempts of cancer cells. The immunodominant glycoprotein gp100_209‐217 epitope, which is liberated from the melanoma differentiation antigen gp100^PMEL17, is part of immunotherapy trials. By analyzing different human melanoma...

article

Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8+ T Cells during Bacterial Infection

Anouk C.M. Platteel, Juliane Liepe, Kathrin Textoris-Taube, Christin Keller, more

Cell Reports > 2017 > 20 > 5 > 1242-1253

Proteasome-catalyzed peptide splicing (PCPS) generates peptides that are presented by MHC class I molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. Here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. Applying this strategy to a murine Listeria monocytogenes infection...

article

Proceedings of the Frontiers of Retrovirology Conference 2016

Irena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, more

Retrovirology > 2016 > 13 > 1 > 1-40

Table of contents Oral presentations Session 1: Entry & uncoating O1 Host cell polo-like kinases (PLKs) promote early prototype foamy virus (PFV) replication Irena Zurnic, Sylvia Hütter, Ute Lehmann, Nicole Stanke, Juliane Reh, Tobias Kern, Fabian Lindel, Gesche Gerresheim, Martin Hamann, Erik Müllers, Paul Lesbats, Peter Cherepanov, Erik Serrao, Alan Engelman, Dirk Lindemann...

chapter

The Dcpm protecting group is a useful alternative to the Hmb residue

Petra Henklein, René Röder, Hardy Weißhoff, Peter Henklein, more

Advances in Experimental Medicine and Biology > Peptides for Youth > Peptide Materials Science > 247-248

article

Proteinase-activated receptor 2 (PAR₂) in hepatic stellate cells – evidence for a role in hepatocellular carcinoma growth in vivo

Franziska Mußbach, Hendrik Ungefroren, Bernd Günther, Kathrin Katenkamp, more

Molecular Cancer > 2016 > 15 > 1 > 1-16

Background Previous studies have established that proteinase-activated receptor 2 (PAR₂) promotes migration and invasion of hepatocellular carcinoma (HCC) cells, suggesting a role in HCC progression. Here, we assessed the impact of PAR₂ in HCC stromal cells on HCC growth using LX-2 hepatic stellate cells (HSCs) and Hep3B cells as model. Methods PAR₂ expression and function in LX-2 cells was analysed...

article

The proteasome immunosubunits, PA28 and ER‐aminopeptidase 1 protect melanoma cells from efficient MART‐1_26‐35‐specific T‐cell recognition

Martin Keller, Frédéric Ebstein, Elke Bürger, Kathrin Textoris‐Taube, more

European Journal of Immunology > 45 > 12 > 3257 - 3268

The immunodominant MART‐1_26(27)‐35 epitope, liberated from the differentiation antigen melanoma antigen recognized by T cells/melanoma antigen A (MART‐1/Melan‐A), has been frequently targeted in melanoma immunotherapy, but with limited clinical success. Previous studies suggested that this is in part due to an insufficient peptide supply and epitope presentation, since proteasomes containing the immunosubunits...

article

The native structure of annexin A2 peptides in hydrophilic environment determines their anti-angiogenic effects

Aase M. Raddum, Hanne Hollås, Igor A. Shumilin, Petra Henklein, more

Biochemical Pharmacology > 2015 > 95 > 1 > 1-15

The progression of aggressive cancer occurs via angiogenesis and metastasis makes these processes important targets for the development of anti-cancer agents. However, recent studies have raised the concern that selective inhibition of angiogenesis results in a switch towards increased tumour growth and metastasis. Since Annexin A2 (AnxA2) is involved in both angiogenesis and metastasis, it may serve...

article

Development of Peptidyl Lysine Dendrons: 1,3‐Dipolar Cycloaddition for Peptide Coupling and Antibody Recognition

Christine Hüttl, Cornelia Hettrich, Melanie Riedel, Petra Henklein, more

Chemical Biology & Drug Design > 85 > 5 > 565 - 573

A straightforward synthesis strategy to multimerize a peptide mimotopes for antibody B13‐DE1 recognition is described based on lysine dendrons as multivalent scaffolds. Lysine dendrons that possess N‐terminal alkyne residues at the periphery were quantitative functionalized with azido peptides using click chemistry. The solid‐phase peptide synthesis (SPPS) allows preparing the peptide dendron in high...

article

Proteinase-activated receptor 1- and 4-promoted migration of Hep3B hepatocellular carcinoma cells depends on ROS formation and RTK transactivation

Franziska Mußbach, Petra Henklein, Martin Westermann, Utz Settmacher, more

Journal of Cancer Research and Clinical Oncology > 2015 > 141 > 5 > 813-825

Purpose There is growing evidence for a role of proteinase-activated receptors (PARs), a subfamily of G protein-coupled receptors, in cancer. We have previously shown that PAR ₁ and PAR ₄ are able to promote the migration of hepatocellular carcinoma (HCC) cells suggesting a function in HCC progression. In this study, we assessed the underlying signalling mechanisms...

article

Proteasome isoforms exhibit only quantitative differences in cleavage and epitope generation

Michele Mishto, Juliane Liepe, Kathrin Textoris‐Taube, Christin Keller, more

European Journal of Immunology > 44 > 12 > 3508 - 3521

Immunoproteasomes are considered to be optimised to process Ags and to alter the peptide repertoire by generating a qualitatively different set of MHC class I epitopes. Whether the immunoproteasome at the biochemical level, influence the quality rather than the quantity of the immuno‐genic peptide pool is still unclear. Here, we quantified the cleavage‐site usage by human standard‐ and immunoproteasomes,...

article

Assembling an ion channel: ORF 3a from SARS‐CoV

Tze‐Hsiang Chien, Ya‐Ling Chiang, Chin‐Pei Chen, Petra Henklein, more

Biopolymers > 99 > 9 > 628 - 635

Protein 3a is a 274 amino acid polytopic channel protein with three putative transmembrane domains (TMDs) encoded by severe acute respiratory syndrome corona virus (SARS‐CoV). Synthetic peptides corresponding to each of its three individual transmembrane domains (TMDs) are reconstituted into artificial lipid bilayers. Only TMD2 and TMD3 induce channel activity. Reconstitution of the peptides as TMD1...

article

HIV-1 p6 — a structured to flexible multifunctional membrane-interacting protein

Sara Marie Øie Solbak, Tove Ragna Reksten, Friedrich Hahn, Victor Wray, more

BBA - Biomembranes > 2013 > 1828 > 2 > 816-823

The human immunodeficiency virus type 1 (HIV-1) p6 protein has recently been recognized as a docking site for several cellular and viral binding partners and is important for the formation of infectious viruses. Most of its known functions are suggested to occur under hydrophobic conditions near the cytoplasmic membrane, where the protein is presumed to exist in its most structured state. Although...

article

Influenza A virus protein PB1-F2 from different strains shows distinct structural signatures

Sara M.Ø. Solbak, Alok Sharma, Karsten Bruns, René Röder, more

BBA - Proteins and Proteomics > 2013 > 1834 > 2 > 568-582

The proapoptotic influenza A virus PB1-F2 protein contributes to viral pathogenicity and is present in most human and avian influenza isolates. The structures of full-length PB1-F2 of the influenza strains Pandemic flu 2009 H1N1, 1918 Spanish flu H1N1, Bird flu H5N1 and H1N1 PR8, have been characterized by NMR and CD spectroscopy. The study was conducted using chemically synthesized full-length PB1-F2...

article

HIV-1 p6—Another viral interaction partner to the host cellular protein cyclophilin A

Sara M.Ø. Solbak, Tove R. Reksten, Rene Röder, Victor Wray, more

BBA - Proteins and Proteomics > 2012 > 1824 > 4 > 667-678

The 52-amino acid human immunodeficiency virus type 1 (HIV-1) p6 protein has previously been recognized as a docking site for several cellular and viral binding factors and is important for the formation of infectious viruses. A particular structural feature of p6 is the notably high relative content of proline residues, located at positions 5, 7, 10, 11, 24, 30, 37 and 49 in the sequence. Proline...

article

Proteinase-activated receptor 2 (PAR₂) in cholangiocarcinoma (CCA) cells: effects on signaling and cellular level

Roland Kaufmann, Alexander Hascher, Franziska Mußbach, Petra Henklein, more

Histochemistry and Cell Biology > 2012 > 138 > 6 > 913-924

In this study, we demonstrate functional expression of the proteinase-activated receptor 2 (PAR₂), a member of a G-protein receptor subfamily in primary cholangiocarcinoma (PCCA) cell cultures. Treatment of PCCA cells with the serine proteinase trypsin and the PAR₂-selective activating peptide, furoyl-LIGRLO-NH₂, increased migration across a collagen membrane barrier. This effect was inhibited by...

article

An optimized MM/PBSA virtual screening approach applied to an HIV‐1 gp41 fusion peptide inhibitor

Tom Venken, Daniela Krnavek, Jan Münch, Frank Kirchhoff, more

Proteins: Structure, Function, and Bioinformatics > 79 > 11 > 3221 - 3235

VIRus Inhibitory Peptide (VIRIP), a 20 amino acid peptide, binds to the fusion peptide (FP) of human immunodeficiency virus type 1 (HIV‐1) gp41 and blocks viral entry. VIRIP derivatives with improved antiviral activity have been developed, and one of those derivatives has recently proven effective and safe in a phase 1/2 clinical trial. Here, molecular dynamics were executed in combination with molecular...

article

Proteinase-activated receptor 2-mediated calcium signaling in hepatocellular carcinoma cells

Roland Kaufmann, Franziska Mußbach, Petra Henklein, Utz Settmacher

Journal of Cancer Research and Clinical Oncology > 2011 > 137 > 6 > 965-973

Purpose The proteinase-activated receptor-2 (PAR₂), a member of a newly discovered G protein–coupled receptor subfamily has recently been shown to promote hepatocellular carcinoma (HCC) cell invasion, suggesting a function in HCC progression. In this study, the effect of PAR₂ on intracellular calcium and its involvement in p42/p44 MAPKinase activation in HEP-3B cells and in two primary HCC cultures...

article

On the use of N‐dicyclopropylmethyl aspartyl‐glycine synthone for backbone amide protection

René Röder, Petra Henklein, Hardy Weißhoff, Clemens Mügge, more

Journal of Peptide Science > 16 > 1 > 65 - 70

To prevent aspartimide formation and related side products in Asp‐Xaa, particularly Asp‐Gly‐containing peptides, usually the 2‐hydroxy‐4‐methoxybenzyl (Hmb) backbone amide protection is applied for peptide synthesis according to the Fmoc‐protocols. In the present study, the usefulness of the recently proposed acid‐labile dicyclopropylmethyl (Dcpm) protectant was analyzed. Despite the significant steric...

article

The ubiquitin- and proteasome-dependent degradation of COX-2 is regulated by the COP9 signalosome and differentially influenced by coxibs

Heiko Neuss, Xiaohua Huang, Bettina K. J. Hetfeld, Rupal Deva, more

Journal of Molecular Medicine > 2007 > 85 > 9 > 961-970

The cyclooxygenase-2 (COX-2) enzyme is induced upon inflammation and in neoplastic tissues. It produces prostaglandins that stimulate tumor angiogenesis and tumor growth. Therefore, destruction and/or specific inhibition of COX-2 should be an important aspect of future tumor therapy. Recently, clinical application of specific COX-2 inhibitors called coxibs became doubtfully because they produce serious...

article

In vitro pharmacological activity of the tetrahydroisoquinoline salsolinol present in products from Theobroma cacao L. like cocoa and chocolate

Matthias F. Melzig, Ingo Putscher, Petra Henklein, Hanka Haber

Journal of Ethnopharmacology > 2000 > 73 > 1-2 > 153 - 159

Cocoa and chocolate contain the tetrahydroisoquinoline alkaloid salsolinol up to a concentration of 25 μg/g. Salsolinol is a dopaminergic active compound which binds to the D ₂ receptor family, especially to the D ₃ receptor with a K _i of 0.48+/-0.021 μmol/l. It inhibits the formation of cyclic AMP and the release of β-endorphin and ACTH in a pituitary cell system. Taking the...

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Search results for: Petra Henklein

ER‐aminopeptidase 1 determines the processing and presentation of an immunotherapy‐relevant melanoma epitope

Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8+ T Cells during Bacterial Infection

Proceedings of the Frontiers of Retrovirology Conference 2016

The Dcpm protecting group is a useful alternative to the Hmb residue

Proteinase-activated receptor 2 (PAR₂) in hepatic stellate cells – evidence for a role in hepatocellular carcinoma growth in vivo

The proteasome immunosubunits, PA28 and ER‐aminopeptidase 1 protect melanoma cells from efficient MART‐1_26‐35‐specific T‐cell recognition

The native structure of annexin A2 peptides in hydrophilic environment determines their anti-angiogenic effects

Development of Peptidyl Lysine Dendrons: 1,3‐Dipolar Cycloaddition for Peptide Coupling and Antibody Recognition

Proteinase-activated receptor 1- and 4-promoted migration of Hep3B hepatocellular carcinoma cells depends on ROS formation and RTK transactivation

Proteasome isoforms exhibit only quantitative differences in cleavage and epitope generation

Assembling an ion channel: ORF 3a from SARS‐CoV

HIV-1 p6 — a structured to flexible multifunctional membrane-interacting protein

Influenza A virus protein PB1-F2 from different strains shows distinct structural signatures

HIV-1 p6—Another viral interaction partner to the host cellular protein cyclophilin A

Proteinase-activated receptor 2 (PAR₂) in cholangiocarcinoma (CCA) cells: effects on signaling and cellular level

An optimized MM/PBSA virtual screening approach applied to an HIV‐1 gp41 fusion peptide inhibitor

Proteinase-activated receptor 2-mediated calcium signaling in hepatocellular carcinoma cells

On the use of N‐dicyclopropylmethyl aspartyl‐glycine synthone for backbone amide protection

The ubiquitin- and proteasome-dependent degradation of COX-2 is regulated by the COP9 signalosome and differentially influenced by coxibs

In vitro pharmacological activity of the tetrahydroisoquinoline salsolinol present in products from Theobroma cacao L. like cocoa and chocolate

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