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A simple and convenient protocol for the cryopreservation of the flounder (Paralichthys olivaceus) sperm was established for ''on the spot'' cryopreservation of large quantities of semen. The use of three cryoprotectants, dimethyl sulphoxide (DMSO), glycerol (Gly) and methanol was tested in the method. The percentage of motile sperm present in semen after it had been frozen and thawed in the presence...
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