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Growth and transcriptional profiles of the deep-sea methanarchaeon Methanocaldococcus jannaschii were studied under sudden up-shifts of temperature and pressure. Application of 500 atm of hyperbaric pressure shifted the optimal growth temperature upwards by about 5°C in a high temperature–pressure bioreactor, and increased the specific growth rate threefold at 88°C. In contrast, pressure-shock from...
A series of five progressively saturated C35 isoprenoids has been identified in cell-free extracts of the deep-sea methanogen Methanococcus jannaschii. Production and relative abundance of the isoprenoids were dependent on culture conditions; significant production occurred in a 16-l fermentor (12-l working volume) and a 2.5-l fermentor (2-l working volume) but could not be duplicated in serum bottles...
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