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Representative and fast monitoring of wastewater influent and effluent biochemical oxygen demand (BOD) is an elusive goal for the wastewater industry and regulatory bodies alike. The present study describes a suitable assay, which incorporates activated sludge as the biocatalyst and ferricyanide as the terminal electron acceptor for respiration. A number of different sludges and sludge treatments...
Activated sludge was successfully incorporated as the biocatalyst in the fast, ferricyanide-mediated biochemical oxygen demand (FM-BOD) bioassay. Sludge preparation procedures were optimized for three potential biocatalysts; aeration basin mixed liquor, aerobic digester sludge and return activated sludge. Following a 24h starving period, the return activated sludge and mixed liquor sludges reported...
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