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A sensitive and convenient flow‐injection chemiluminescence (FI‐CL) turn‐on assay for alkaline phosphatase (ALP) activity without any label and synthesis is developed. Cu2+ can catalyze the luminol–H2O2 CL reaction. Pyrophosphate (PPi) can chelate Cu2+ and therefore the Cu2+‐mediated luminol‐H2O2 CL reaction is inhibited. The addition of ALP can catalyze the hydrolysis of PPi into phosphate ions,...
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