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Tigloyl-CoA:pseudotropine acyl transferase esterifies the 3β-hydroxyl group of pseudotropine (3β-hydroxytropane) with the tiglic acid moiety of tigloyl-Coenzyme A. This enzyme was purified to near electrophoretic homogeneity--about 330-fold--from transformed root cultures of Datura stramonium. The protocol involved the sequential use of ammonium sulphate precipitation, hydrophobic interaction, anion-exchange,...
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