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As a long non‐coding RNA, C5orf66‐AS1 is located at 5q31.1. Downregulation and aberrant hypermethylation of C5orf66‐AS1 have been detected in a limited several tumors. However, the biological role and distribution of methylated CpG sites of C5orf66‐AS1 in gastric cardia adenocarcinoma (GCA) development and prognosis are poorly clarified. The present study was to investigate the expression status...