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Classical major histocompatibility complex (MHC) class II molecules play an essential role in immune system. In this study, MHC IIα (Pf‐MHC IIα) and MHC IIβ (Pf‐MHC IIβ) homology genes from pufferfish (Takifugu obscurus) were cloned and their functional characterization in response to bacterial challenge was identified. The nucleotide sequences of the open reading frames (ORFs) of pufferfish Pf‐MHC IIα...
A new cell line was established from the brain of a cultured fish, tilapia (Oreochromis niloticus), designated as TA‐02 (Tilapia Astrocyte clone 02 cell line). The TA‐02 cells are grown for 300 days in an L‐15 medium supplemented with 10% fetal bovine serum (FBS). This cell line showed excellent proliferative capacity and expressed various neuroglial cell markers, including SOX2, SOX10, Hes1, Notch1,...