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Wild-type actinoporins StnI and StnII from the sea anemone Stichodactyla helianthus, as well as their NH 2 -terminal six-His tagged versions, have been overproduced in Escherichia coli. Overproduction of both wild-type proteins was only possible after introducing silent mutations within the 5′-end of their original cDNA sequences. These mutations would prevent the formation of RNA secondary...
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