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Current approaches to measure the activities of microbial extracellular enzymes in aquatic environments are hampered by slow throughput or by differences between the structure of simple substrate proxies and macromolecules. Here we show that measurements of fluorescence anisotropy can be used to determine the hydrolysis rate of two fluorescently labeled polysaccharides, laminarin and xylan, in environmental...
Using a novel multi-cell continuous flow assembly, we studied the dynamic interactions between high-molecular weight substrate consumption, fermentation, and terminal metabolism by microbial communities in anaerobic marine sediments. This system allowed partial physical separation of individual steps in the remineralization of high-molecular weight organic matter: degradation of high-molecular weight...
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