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In this study, graphene oxide‐octadecylsilane incorporated monolithic nano‐columns were developed for protein analysis by nano liquid chromatography (nano LC). The monolithic column with 100 μm id was first prepared by an in situ polymerization using ethylene dimethacrylate (EDMA), 3‐chloro‐2‐hydroxypropylmethacrylate (HPMA‐Cl), and methacryloyl graphene oxide nanoparticles (MGONPs). MGONPs were synthesized...
A carboxy precursor monolithic column, namely poly(carboxy ethyl acrylate‐co‐ethylene glycol dimethacrylate) was first produced in a 100 μm i.d. fused‐silica capillary and subsequently surface bonded with n‐octadecyl (C18) ligands by a post‐polymerization functionalization process with octadecylamine in the presence of N,N´‐dicyclohexylcarbodiimide. The bonding of octadecyl ligands was achieved via...