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Methods. SV40-immortalized human corneal epithelial (HCE-2) cells were co-incubated with trophozoites or lysate of pathogenic Acanthamoeba castellanii (ATCC 30868) in peptoneyeastglucose (PYG) medium. Control cells were exposed to PYG only or extracts similarly prepared from fibroblasts. At 6, 12, and 18h of incubation with trophozoites, HCE-2 cells were examined by light and scanning electron microscopy...
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