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We demonstrate a novel bio‐spectroscopic technique, “simultaneous Raman/GFP microspectroscopy”. It enables organelle specific Raman microspectroscopy of living cells. Fission yeast, Schizosaccharomyces pombe, whose mitochondria are green fluorescence protein (GFP) labeled, is used as a test model system. Raman excitation laser and GFP excitation light irradiate the sample yeast cells simultaneously...
The ‘Raman spectroscopic signature of life’ is a Raman band at 1602 cm−1 that sharply reflects the metabolic activity of cell mitochondria. Here we report the study of thissignature in isolated yeast mitochondria. The existence and behaviour of the 1602 cm−1 band in isolated mitochondria have been confirmed to be the same as in living yeast cells: the intensity of the band decreases with timewhen...
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