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Recent Raman and Raman optical activity (ROA) results have demonstrated that dimethyl sulfoxide (DMSO) induces the selective conversion of α‐helix motifs into the poly(L‐proline) II (PPII) helix conformation in an array of proteins, while β‐sheets remain mostly unaffected. Human serum albumin (HSA), a highly α‐helical protein, underwent the most dramatic changes and, therefore, was selected as a model...
Raman spectroscopy is being increasingly applied to the investigation of conformational transitions in polypeptides and proteins, and in particular protein unfolding, due to its ability to monitor changes in secondary structure. Here we focus on the application of two different two-dimensional correlation methods, autocorrelation and moving windows techniques, to investigate the pH-induced conformational...
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