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We introduce a new model-based approach for automatic quantification of colocalizations in multichannel 3D microscopy images. The approach uses different 3D parametric intensity models in conjunction with a model fitting scheme to localize and quantify subcellular structures with high accuracy. The central idea is to determine colocalizations between different channels based on the estimated geometry...
In cell biology, modern imaging techniques using fluorescence microscopy allow to visualize specific nuclear structures in situ in the same cell nucleus. Hence, distances between these structures can be evaluated, in particular co-localization can be investigated. When the nucleus alters its global shape, especially if the structures are imaged sequentially, the distances are changing as well and...
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