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Microbial ∆1-dehydrogenation is one of the most important transformations in the synthesis of steroid hormones. In this study, a 3-ketosteroid-∆1-dehydrogenase (kstDF) involved in fusidane antibiotic biosynthesis from Aspergillus fumigatus CICC 40167 was characterized for use in steroid transformation. KstDF encodes a polypeptide consisting of 637 amino acid residues. It shows 51% amino acid identity...
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