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Effective expression of porcine interferon-α (pIFN-α) with recombinant Pichia pastoris was conducted in a bench-scale fermentor using an in situ methanol electrode-based feeding process with the control level of methanol concentration linearly increased to 10 g l−1 for the first 20 h and maintained at 10 g l−1 for the rest of expression phase. With this two-stage control process, the highest pIFN-α...
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