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In order to reveal the molecular mechanisms and the metabolic pathway of producing bioflocculant, the changes in protein complexes of F2 grown in minimal media versus bioflocculant media and those of F6 grown in minimal media versus bioflocculant media were respectively researched by SDS-PAGE. SDS-PAGE results indicated that the relative band volumes of three bands were obviously decreased in F2,...
The strain F2 which produce excellent flocculating substance were isolated from soil samples and identified as Rhizobium radiobacter. The isolates F2 produced the flocculating substance during the late logarithmic growth phase and the early stationary growth phase. It was founded that the flocculation activated substances of BF distributed mainly in upper phase and were mainly composed of polysaccharide...
Polysaccharide is the main component of the flocculation activated substances produced by bioflocculant-producing bacterium F6. It could be extracted from the supernatant of the fermented medium by cold acetone precipitation and purified by treating with Sevag reagent. The result showed that the optimum extracting conditions were that concentrating multiple is 1/3, acetone consistency is 100%, acetone...
Two strains (designated F2, F6) which produce excellent flocculating substance were isolated from soil samples and identified as Rhizobium radiobacter and Bacillus sphaeicus. The compound bioflocculant (CBF) with higher flocculating activity was obtained by coculturing F2 and F6. The isolates F2 and F6 produced the flocculating substance during the late logarithmic growth phase and the early stationary...
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