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One of the main challenges for laser‐scanning microscopy of biological tissues with refractive heterogeneities is the degradation in spatial resolution that occurs as a result of beam steering and distortion. This challenge is particularly significant for dual‐axis confocal (DAC) microscopy, which achieves improved spatial‐filtering and optical‐sectioning performance over traditional confocal microscopy...
We utilize both Gaussian and Bessel illumination in a point-scanned dual-axis confocal (DAC) microscope and quantify the resultant degradation of resolution when imaging in phantoms and tissues. Results indicate that Bessel illumination exhibits reduced resolution degradation from micro-scale refractive heterogeneities compared to conventional Gaussian illumination.
We present a strategy to preserve contrast over a deeper range of imaging depths in the context of confocal microscopy using low-power (0.5 mW) diode laser illumination. While the dual-axis confocal microscope architecture's intersecting illumination and collection beams significantly improves the spatial-filtering and optical-sectioning performance of confocal microscopy, we propose that modulating...
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