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Hair cortisol has been used as a biomarker of chronic stress. The detected contents of hair cortisol might depend on the incubation duration in solvents for no-milled hair samples with 3-layer structure. However, there was no research on the dissolution mechanism of hair analytes.After uniform mixture, no-milled hair samples were incubated in methanol and water for the 12 different durations and milled...
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