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Biodegradable polymer polycaprolactone (PCL; Mw~100,000) fiber mats containing shikonin from the plant Lithospermumerythrorhizon, widely known for its wound healing, anti-tumor, antioxidant, and anti-inflammatory properties, were prepared via electrospinning method. Incorporation of shikonin in the neat PCL solution did not affect the morphology of the resulting fibers, as both the neat and the shikonin-loaded...
Novel membranes were prepared through covalently coupling of chitosan (CS) to activate nylon membrane after the reaction of the microporous nylon membrane with formaldehyde. Non-specific adsorption on the CS-coated nylon membrane decreased greatly. Yellow 3G-P/Reactive Red 120 were then covalently immobilized onto composite membrane as dye ligands. These Red 120/Yellow 3G-P-CS-membranes were used...
The behavior of bromelain adsorption on the dye- ligand affinity membrane was investigated at 277,298 and 310 K, which was chitosan (CS) coated to decrease the non-specific adsorption of protein. Reactive Blue 4 was immobilized onto the CS-coated nylon membrane as an affinity ligand. In order to examine the rate limiting step of Reactive Blue 4-immobilized composite membrane adsorption, the two adsorption...
This study demonstrates that Aminoethylisothiourea (AET), a potent inhibitor of inducible nitric oxide synthase (NOS), is an irreversible competitive inhibitor of the diphenolase activity of mushroom tyrosinase when L-DOPA was assayed spectrophotometrically in vitro. The inhibition of diphenolase activity of tyrosinase by AET showed positive kinetic cooperativity which arose from the protection of...
Papain (EC 3.4.22.2) has been chemically modified using two reagents including different anhydrides of maleic and poly-maleic acids. The average ratio of modified-NH2 was tested by trinitrobenzenesulfonic acid (TNBS) method. The native and modified papain were purified by dialysis and their structures were characterized by FTIR. The factor related with the activity of the modified papain, such as...
Nylon membranes are first hydrolyed with dilute HCl and then treated with chitosan before being used as the affinity carrier. Papain as a ligand has been immobilized on the activated membranes with glutaraldehyde as a crosslinking agent. The factors involving with the activity of immobilized papain, such as concentration of glutaraldehyde, pH, temperature, reaction time, and the amount of added papain...
It is commonly recognized that damage to bone tissue begins to occur in response to these supra-physiologic mechanical loading. Parathyroid hormone (PTH) plays a key role on the anabolic effects of osteoblast. However, the effects of N-terminal 1-34 fragment of human PTH (hPTHl-34) on osteoblast under supra-threshold mechanical loading are unknown. We recently used a stretch-induced from the hypotonic...
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