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A real-time PCR assay based on the TaqMan chemistry was developed for reliable and quantitative detection of CD81 in Tupaia belangeri. The assay is performed with the ABI 7300 system using TaqMan probe and primers amplifying 118bp CD81 fragment of Tupaia belangeri. The standard curve for quantitation of target gene showed linearity over an at least 5-log DNA concentration range, represents 103 to...
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