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The proteins from the brain tissue of the fish Paralichthys olivaceus (PO) were separated by the optimized two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Approximately 650 protein spots were detected with 200 μg of protein load in each SDS-PAGE gel, and the pH varied from 3.0 to 10.0. The differential proteins were separated and identified by proteomic technologies, and further selected...
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