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A triple-resonance pulse sequence is presented for the quantitative measurement of 1 H α – 13 C α single-bond couplings in 15 N, 13 C uniformly labeled proteins. This 1 J CH -modulated (HACACO)NH experiment yields 1 H N – 15 N-correlated 2D spectra in which the amplitude of each peak is modulated by the 1 ...
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