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This paper described a novel method to immobilize trypsin on IAM. The immobilization procedures involved the following steps: IAM was packed into the fused silica capillary (400 mum I.D.times690 mum O.D.times5 cm length); subsequently, trypsin was dynamically immobilized on IAM by injecting 1 mg/ml trypsin aqueous solution using the frontal chromatography. The activity of trypsin-micro-IMERs was on-line...
Monoamine oxidase (MAO) immobilized in the immobilized artificial membrane (IAM) was packed into a capillary to make a chromatography column to simulate the MAO function of catalyzing the oxidative deamination of amine in organism. MAO marked with quantum dots (QDs) was used as probe to demonstrated that MAO was immobilized in the IAM hydrophobic interface, and the enzymatic activity analysis proved...
A new fluorescence imaging method was used for the characterization of the immobilized pH gradient formed in capillary, which was based on charge carried quantum dots (QDs). It was found that the positive charged QDs were concentrated in the same positive region in capillary after CIEF, no matter which terminal of the capillary was used for injection. Furthermore, the formation of the pH gradient...
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