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Scalable, low-latency and accurate RFID counting algorithms have recently been proposed as a fundamental building block to support more complex query operations in a large-scale RFID system. One distinct feature of these algorithms is that they do not require explicit identification of individual tags and therefore can eliminate the latency bottleneck caused by serialization during multiple access...
Immunoassay is one of the most important protein detection methods, which is widely used in clinical diagnosis, environment monitor and biological research due to its high specificity and sensitivity. Most of the existing protein immunoassay detection devices have the weakness of high expenses, large scale and low sample processing rate. Microfluidic immunoassay chips have been widely recognized as...
In this study, the copper target ion beam sputtering deposition (CTIBSD) method was used for improving the thermal performance of commercial ceramic peltier and several PCR chips were made of NOA81 in few minutes by UV soft lithography technology. The thermal uniformity and heat transfer rate were simulated by Comsol to investigate the improved effect of coppering peltier. In addition, the thermal...
In this study, an integrated multidimensional nano-flow liquid chromatography platform was established with the combination of peptide separation via online SCX, and successfully applied for proteome analysis. By this platform, peptides were first separated by a SCX microcolumn under a series of salt steps, separated by nano-reversed phase liquid chromatography, and finally identified by ESI-MS/MS...
Microfluidic chip has been an important tool for the study of life science, especially in cell biology researches. The chip provides precise manipulation of cells and helps establish similar environment in body network. A promising material, Noland Optical Adhesive 81 (NOA 81), was adopted here to fast fabricate the chip. The material was first cured and used for cell culture directly to test the...
Three kinds of chromatography materials with temperature response have been prepared, and they were characterized by IR and TGA. By using the three materials, we studied the separation performance of four different steroids under 5 different temperatures. The results showed that bigger grafting density would lead to shorter chain, lower temperature response and longer retention time. Thus, it is important...
In present study, three kinds of temperature-responsive chromatography materials with different graft densities under the same graft chain length were synthesized via Atom transfer radical polymerization (ATRP), and they were characterized by Infrared detection and Thermo gravimetric analysis (TGA). Furthermore, the influences of temperature and graft density on the separation performance of steroidal...
Parkinson's disease (PD) is a common neurodegenerative disorder in aging and loss of dopamine (DA) in nigrostriatal system is an important character of PD. Animal models is very useful in the research of pathogenesis of PD. However, the DA content is not always decreased in the nigrostriatal system of these models as the situation of PD patients. Viral vector-based PD models in animals were newly...
To solve the problem of stability and accuracy of haptic feedback output in virtual surgery. We have made thorough study of soft tissue deformation with phantom desktop in virtual scene and systems analysis of factors which effect on soft tissue deformation. First of all, a haptic feedback model is constructed, which could express normal direction and surface friction as well as the tactile texture...
Objective: Oxidative stress is a candidate mechanism for ethanol neuropathology in fetal alcohol spectrum disorders. Our overall objective was to investigate the hypothesis that ethanol neurotoxicity involves production of reactive oxygen species (ROS) with negative downstream consequences for mitochondrial membrane potential and neuron survival. Methods: We use P4 cerebellar granule neurons (CGNs)...
Using the technology of molecular docking, the inhibitor structural features of human epidermal growth factor receptor-2 were studied by screening human epidermal growth factor receptor-2 inhibitors from natural products databases. DS virtual screening systems had been established. The extracellular region and kinase domain of HER2 were selected as target protein. Different conformations, interaction...
Semicarbazide-sensitive amine oxidase (SSAO) is multifunctional enzyme which is highly express in vasculature, smooth muscle and adipose tissues. Clinical and experimental studies suggest that increased activity and the catalytic product of deamination may be toxic and cause the tissue damage. Correlation between the SSAO activity and severity of different serious pathological condition such as Diabetes,...
Evidence shows that non-enzymatic glycation plays an important role in the development of diabetes, diabetes-related complications and neurodegenerative diseases. Mass spectrometric methods have been used for non-enzymatic glycation of proteins. At present, CID remains the major fragmentation method for peptide sequencing. In this study, we utilized synthesized peptide models to study fragmentation...
Post-digestion 18O labeling technique and comparative proteomics are used to carry out a large-scale proteome characterization for α-synuclein overexpression transgene mouse model. Proteins of transgene mice brain were labeled by 18O, but not for proteins of control. Spectrum Mill software give the specific value of H/L of every protein detected by HPLC/ESI-QTOF, which reflect the difference of protein...
Parkinson's disease (PD) is a multifactorial, neurodegenerative disease where etiopathogenesis are not fully understood. Mutations in α-Synuclein (α-Syn) were the first genetic defect linked to PD. They are deposited in Lewy bodies (LBs) characteristic for PD. Some experiments had showed that A30P mutant a-Syn have high toxicity than wide-type α-Syn. Here we used A30Pα-Syn transgenic mice model to...
Parkinson's disease (PD) is a common neurodegenerative disease. The prevalence of PD among people over 65 years old is about 2 % and it is induced by multifactor including age, gene and environment. Recently, proteomics are increasingly being applied to identify biomarkers for disease. In this study, unilateral 6-hydroxydopamine (6-OHDA) in striatum of PD rat model was established first and demonstrated...
A system of the signal acquisition and data processing used for light-emitting diodes induced fluorescence (LED-IF) detection in our laboratory was described in this paper. A high-speed data acquisition module was developed. The function of signal acquisition in real-time and data processing was realized utilizing the graphical programming environment LabVIEW. To reduce the distorted come from the...
Aptamers are single-stranded oligonucleotides that bind with high specificity and affinity to a target molecule. Aptamer-based biosensors have become a new class of biosensors, which utilized aptamers as highly selective recognition elements as a result of good performance in the capture of target analytes. In this study, aptamer was used as an recognition element of biosensor, and was integrated...
Several new RFID counting algorithms have recently been proposed based on the probabilistic counting schemes introduced by Kodialam et al. These existing algorithms took into account the unreliability of the communication channels between the RFID reader and the tags, and are capable of providing accurate tag-count estimates. However, all algorithms were designed and evaluated based on a simplistic...
Along with the durative change of global climate, natural disasters are becoming more and more frequent. China is one of the countries which have the most serious natural disasters. Although the government has played an irreplaceable role in the management of natural disaster crises, the pattern of sole government rescues cannot obtain the optimal effect. Corporate social responsibility in natural...
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