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A rise in [Ca 2+ ] i provides the trigger for neurotransmitter release at neuronal boutons. We have used confocal microscopy and Ca 2+ sensitive dyes to directly measure the action potential-evoked [Ca 2+ ] i in the boutons of Schaffer collaterals. This reveals that the trial-by-trial amplitude of the evoked Ca 2+ transient is bimodally distributed....
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