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The active site of flavoprotein oxidases frequently contains two active-site tyrosines, e.g., Y223 and Y238 in d-amino acid oxidases (DAAO) from Rhodotorula gracilis. In the past, these residues were individually mutated to phenylalanine and serine. To further study their role we undertake an interpretation of Y223F–Y238F double mutation. The spectral properties of the double mutant are similar to...
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