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To determine the effect of vitrification and 5-aza-2′-deoxycytidine (5-aza-dC) on the methylation levels of the putative imprinted control region (ICR) of H19 and H19 expression in bovine two-cell embryos and their derived blastocysts.Experimental animal study.Academic institution.Abattoir-derived bovine ovaries.Vitrified two-cell embryos were cultured in vitro to blastocysts with 0.01 μM 5-aza-dC...
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