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This report showed some physico-chemical properties and structural features about UL41 protein predicted by some software and on-line tools. The analysis showed both the signal peptide and the trans membrance region are not found. Meanwhile, the protein has twenty-four potential phosphorylation sites. In addition, the protein has more hydrophilic amine acid districts than hydrophobic districts and...
The Duck Plague Virus (DPV) is the causative agent of Duck plague, which causes significant economic losses in the commercial duck industry all over the world. Our laboratory had identified and sequenced the DPV UL41 gene. Bioinformatics analysis of DPV UL41 gene were performed by some software and online tools. The results showed that GC content of duck plague virus UL41 gene was 42.42%, which encoded...
Glycoprotein K encoded by the DEV-UL53 gene is one of the DEV envelope glycoproteins. Like most members of the alphaherpesvirinae, the genes in the UL region of its genome are well conserved, which means that the major DEV envelope glycoprotein K possessing similar functions of envelope glycoprotein K of alphaherpesvirinae plays an essential role in viral replication and cell fusion. Glycoprotein...
The glycoprotein I (gI) gene homologue of duck plague virus (DPV) was cloned by degenerate polymerase chain reaction (PCR) and sequenced. DPV gI gene open reading frame (ORF) was 1116 bp in length and its primary translation product was a polypeptide of 371 amino acids long. Comparison with other herpesvirus revealed higher similarity, had a close evolution relationship with members of the genus Mardivirus...
The DPV UL46 gene( GenBank accession No. EU195108) isolated by our laboratory was amplified by PCR and cloned into pMD18-T vector. The recombinant plasmid pMD18-T-UL46 was identified by PCR, restriction enzymes (BamHI+XhoI), sequencing and nucleic acid dot-hybridization. Then the gene was analyzed by bioinformatics tools. The results showed that a 2220bp complete open reading frame of 739 amino acids...
Abstract-An open reading frame (ORF) containing 675 nucleotides was got by sequencing the DEV gene libraries constructed by our laboratory. The ORF was identified as DEV UL45 gene by aligning with gene bank database using the software of BlastN and ORF Finder. Specific primers were designed and PCR products containing this ORF were cloned into the vector of PMD18-T. Dot blot confirmed the UL45 gene...
The UL27 open reading frame of the Duck Plague virus encodes glycoprotein B, the molecular characteristic of this gene was analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 1000 putative proteins, contains the conserved domain of the Herpesvirus UL27-like protein. Similarity comparisons of DPV CHv strain gB protein sequences with those of 20 known herpesviruses...
The focus of this paper is on the newly identified UL55 gene of DEV in our laboratory.Available bioinformatic tools were used to describe its molecular characteristics. A identified nucleotide sequence about 561bp of DEV UL55 ,consisting a complete ORF, was deduced to encode a protein with 186 amino acids.The 20.7981 kDa protein whose singnal peptide and transmembrane region are both absent. Codon...
The US4 open reading frame of the Duck enteritis virus (DEV) encodes glycoprotein G, a protein comprising 459 amino acids with a putative molecular mass of 50.58 kDa. The characteristics of this protein were analyzed through bioinformatics softwares and tools. Glycoprotein G sequence analysis indicates that the protein possesses typical characteristics of membrane glycoproteins containing two transmembrane...
A 1296-bp open reading frame (ORF) of duck plague virus (DPV) was cloned and sequenced. Nucleotide sequence analysis revealed that the complete ORF was supposed to be the DPV gC gene (GenBank accession no EU076811), which theoretically encoded a protein comprising 431 amino acids with a putative molecular mass of 45 kDa. Multiple sequence alignment and phylogenetic analysis showed that the DPV gC...
The analysis on codon usage bias of gI gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and molecular characteristic of DEV, and for selecting appropriate host expression systems to improve the expression of target gene. A comparative analysis of the codon usage bias of the DEV gI gene and 22 other refrence herpesviruses gI-like genes was performed, by using the...
A 831-bp complete open reading frame of the duck plague virus UL34 gene (GenBank accession no, EF643562) was identified in our laboratory. The molecular characteristic of this gene was amplified and analyzed with bioinformatics software. The results indicated that this gene encodes an estimated 276 putative protein, contains the conserved domain of the Herpesvirus-UL34-like protein. Phylogenetic tree...
The Duck Plague Virus (DPV) gE gene was identified by constructing the DPV genomic library, and we analyzed the codon usage of DPV gE gene by using CAI, CHIPS and CUSP program of EMBOSS. The results showed that codon usage bias in the DPV gE gene was strong bias towards the synonymous codons with A and T at the third codon position. The amino acids codon usage bias in the DPV gE gene was compared...
The analysis on codon usage bias of UL26.5 gene of duck enteritis virus (DEV) may provide a basis for understanding the evolution and pathogenesis of DEV and for selecting appropriate host expression systems to improve the expression of target genes in vivo and in vitro. In this study, the synonymous codon usage in the UL26.5 gene of DEV and 27 reference herpesviruses have been investigated . The...
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