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Techniques for monitoring cell cultures and fermentation processes not only enable prompt feedback to variations in critical parameters (e.g., media composition and metabolites) but further improve our understanding of the processes themselves. In this context, surface plasmon resonance (SPR) spectroscopy is one of the methods of choice. This technique exploits angle shifting to follow molecular interactions...
The development of a surface plasmon resonance (SPR) spectrometer comprising angular‐resolved analysis for quasi‐monochromatic illumination is reported. The optical system utilizes disposable, injection‐molded chips combined with a lateral imaging optical system for parallel analysis of one‐dimensional spot arrays. Further parallelization is achieved by introducing a segmented light source. This source...
The detection of DNA hybridization in medical diagnostics ought to be rapid, sensitive and specific. A platform technology based on surface plasmon resonance (SPR) is presented. We use TOPAS® chips with integrated optics and combined with microfluidics. Applying a nanoliter dispenser, thiol-modified single-stranded probe DNA (anti-tag) is deposited on the gold surface of the chips to create...
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