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A hierarchically nanostructured organic–inorganic hybrid substrate comprising randomly oriented ZnO nanorods on glass slide with coaxially tethered dense polymer brush, POEGMA‐co‐GMA is reported for highly sensitive antibody microassay, achieving excellent detection specificity, and superior detection limit of as low as 100 fg mL−1 for biomarkers in human serum within a 1 h assay time.
Current high-throughput antibody microarrays greatly rely on enzymatic reactions- or nanostructured tags-based reporter-amplifications for required sensitivity, but they need tedious operation, additional expensive reagents and microwells or microchannels to eliminate crossover interferences, thus resulting in low array density and high expense. A unique ZnO nanorods-grown substrate is developed here...
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