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AimsTo develop a microplate‐based high‐throughput assay for the detection of succinic acid, which is widely used in many fields of industry. The assay can also be applied to screen high succinic acid‐producing strains.
Methods and ResultsHere, we developed a double‐enzyme‐based succinic acid detection assay using fumarate reductase and peroxidase (named FRDP assay) to screen and isolate high succinic...
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