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Salinity tolerance of embryos of obscure puffer Takifugu obscurus was investigated at salinities of 0, 4, 8, 12, 16, 20, 24, 28, and 32 parts per thousand (ppt), respectively. Survival rates of embryos at 0, 4 and 8 ppt salinities were higher than 90% throughout the experiment, and there were no significant differences between 0 and 8 ppt (P>0.05), whereas most embryos died gradually at salinity...
Artificially fertilized eggs of obscure puffer Takifugu obscurus were obtained by induced spawning of cultured broodstock and incubated at temperatures of 15, 19, 23, and 27 °C. The results showed that the optimal temperature for obscure puffer embryonic development ranged from 19 to 23 °C, based on total hatch rate, viability of newly hatched larvae 24 h post-hatch, and total mortality rate of eggs...
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